Ethylene Glycol is a key component in most antifreeze products. The ingestion of ethylene glycol can quickly poison an animal and lead to irreversible liver and kidney damage if poisoning is not determined to have occurred and is not quickly treated. If ethylene glycol is shown to be present in an animal’s blood, treatment is relatively simple.

Catachem has developed three different tests to determine ethylene glycol levels:

Quantitative Test (C504-0A):

Catachem has developed an accurate quantitative test (C504-0A) that determines ethylene glycol levels. Values obtained on Catachem’s quantitative test reagent compare closely to values obtained on GC/MS methods (Am.J.Clin.Pathol. 2011, 136:165-6).

Catachem’s product has been designed to effectively eliminate interferences from propylene glycol, a secondary component in some antifreeze products and also a chemical used as an additive to some veterinary drugs and foodstuffs. Neither Diethylene Glycol or the antidote 4-methylpyrazole (fomepizole), the latter tested to a level of 30 g/L, showed any interference in the assay.


Qualitative Test (C504-0B):

Catachem’s qualitative in-office test requires no instrumentation and uses the same enzymatic technology as its quantitative partner. Although it is subject to somewhat more interference, the test offers the veterinarian a useful tool in an emergency, time-sensitive situation.

The test kit comes with all components needed to evaluate whether an animal has ingested ethylene glycol. Each test uses a parallel control to ensure that reading errors are eliminated and each test kit comes with all the components required to carry out three individual tests. No interference was found from 4-methyl pyrazole (Fomepizole), the common antidote, which was tested to a level of 3,000 mg/dL (30 g/L).

FasTox Test Kit (C504-0C):

Designed for the low volume user for use on a simple spectrophotometer (340 nm wavelength), this product provides three semi-quantitative tests each with low and high controls. Each test is designed for one time use directly off the shelf with a shelf life of approximately 18 months. Mirroring Catachem’s quantitative test formulation, it is not subject to common interferences like propylene glycol. The format of the kit involving the use of both low and high controls during each test run eliminates any need for additional calibration.


For more information on our Ethylene Glycol assays, please view our brochure or contact us.