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VETSPEC™REAGENTS - Chloride

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Chloride in serum using manual or automated applications.

Method Principle

Chloride ions present in the serum sample interact with Ferric ions in dilute acid to form a yellow chloro-complex with maximum absorption at 340 nm. The intensity of the color thus produced is directly proportional to the concentration of the chloride ion in the serum sample. The reaction scheme below illustrates the reaction that occurs in this method.

H+
Fe3+ + 6Cl- ──────> 3Fe 6(Cl)


Preparation Of Working Reagents

Catachem Chloride 340 nm reagents are packaged in ready-to-use form. No preparation is required.

Reagent Storage And Stability

Store Catachem Chloride 340 nm reagents at room temperature. When stored as directed, the reagents are stable until the expiration date stated on the label.

Method Performance Characteristics

Sensitivity: The sensitivity of this method is 0.004 - 0.0045 absorbance units per mEq/L.
Linear Range: In this method there is no significant nonlinearity over the range of 0-150 mEq/L.
Precision: Within-run and day-to-day precision is summarized below.

Chloride

Within-Run Precision

Total Precision

MEAN

SD

CV

SD

CV

mg/dL

mg/dL

%

mg/dL

%

77

0.83

1.10

1.07

1.40

96

0.84

0.87

1.70

1.20

120

1.26

1.40

1.61

1.30


Correlation

A comparison of this method using Catachem’s Mercuric Thiocynate Chloride method on an automated analyzer  resulted in the following regression statistics.

Correlation Data

Parameter

Data Observed

N

50

Range

90 - 110 mEq/L

Regression

1.14x + 18.4

Correlation

  0.987

Sy,x

1.6