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VETSPEC™ REAGENTS - Sorbitol Dehydrogenase (SDH)

 

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Sorbitol Dehydrogenase in serum using manual or automated applications.

Method Principle

Several procedures have been reported in the literature for measuring the enzyme, L-iditol Dehydrogenase or Sorbitol Dehydrogenase (SDH, EC 1.1.1.14) in serum. The Catachem procedure is based on the enzymatic procedure described by Clive I. Rose and Arthur R. Henderson. In this procedure, SDH catalyzes the reversible oxidation-reduction reaction between Sorbitol and D-Fructose with concomitant oxidation of NADH to NAD+. The decrease in absorbance is monitored at 340 nm. The delta absorbance produced is directly proportional to the concentration of SDH activity in the serum sample. The reaction scheme below illustrates the reactions that take place in this SDH procedure.

SDH
D-Fructose + NADH ─────> D-Sorbitol + NAD+

 

Reagent Storage And Stability

Store the SDH Sample Diluent (R1) and the SDH Activator Reagent (R2) at 2-8°C. When stored as directed these reagents are stable until expiration date stated on the label.

Working Reagent Preparation

The SDH Sample Diluent Working Reagent and Activator Working Reagent (R2) are ready for use. No preparation is required. Upon opening the Working Reagents are stable for 60 days at 2-8°C.

Method Performance Characteristics

Sensitivity: Using a path length of 1 cm, a delta absorbance of 0.0016-0.0024 per mg/ml should be obtained.
Linearity: In this procedure there is no significant nonlinearity over the range of 0-100 U/L.

SDH

Within-Run Precision

Total Precision

MEAN

SD

CV

SD

CV

U/L

U/L

%

U/L

%

6.0

0.438

7.45

 

 

15.0

0.521

3.36

 

 

31.0

0.386

1.91

 

 

 

Correlation Data

Parameter

Data Observed

N

12

Range

4.1-471

Mean Y

41.88

Mean X

43.84

Regression

Y  = 0.959x - 0.16

Correlation

r = 0.978

Sy,x

11.29