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VETSPEC™ REAGENTS - Lactate

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Lactate in serum using manual or automated applications.

Method Principle

Lactate Oxidase catalyzes the oxidation of Lactic Acid to Pyruvate and Hydrogen Peroxide. The Hydrogen Peroxide produced is quantitatively determined by coupling 4-aminoantipyrine with TOOS where a Quinoneimine dye with maximum absorption at 550 nm is produced. The intensity of the dye color thus produced is directly proportional to the concentration of Lactic Acid in the serum sample. The reaction scheme below illustrates the reactions that occur in this method.

Lactate Oxidase
  Lactic Acid + O2 ─────────────> Pyruvate + H2O2


            Peroxidase
2H2O2 + 4-aminoantipyrine + TOOS ────────> Quinoneimine dye + 4H2O
 

Reagent Storage And Stability

The Lactate reagent is stored at 2-8°C. When stored as directed the reagents are stable until  the expiration date stated on the label.

Working Reagent Preparation

Catachem Lactate reagents are packaged in ready-to-use form. No preparation is required. Upon opening the Working Reagent is stable for at least 60 days stored at 2-8ºC.

Method Performance Characteristics

Sensitivity: Using a path length of 1 cm, a Δ-absorbance of 0.03-0.028 per g/dl should be obtained.
Linearity:  This procedure is linear over the range of 0-25 mmol/L.
Precision: Precision data was obtained using three levels of commercially available controls and following the NCCLS EP5-T2 procedure (7). The following results were observed.

Lactate

Precision

Within-Run

Total

MEAN

SD

CV

SD

CV

mmol/L

mmol/L

%

mmol/L

%

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Correlation

A comparison of Catachem Lactate method using an automated analyzer was carried out against a reference Lactate procedure based upon the lactate to pyruvate reaction. The following regression statistics were observed:

Correlation Data

Parameter

Data Observed

N

 

Range (U/L)

 

Regression

 

Correlation

 

Sy,x