#
DISCRETPAK™REAGENTS - Albumin

##
Intended Use

For **IN VITRO** quantitative determination of **Albumin** in serum or plasma using manual or automated applications.

##
Method Principle

BCG dye at a pH of 4.2 and in the presence of a non-ionic surfactant Brij-35 specifically binds albumin to form a colored BCG-Albumin complex with a maximum absorbance at 630nm. The increase in absorbance is directly proportional to the Albumin concentration present in the sample.

pH 4.2

Albumin + BCG^{ } ──────> BCG-Alubmin Complex

Brij-35

##
Method Performance Characteristics

**Sensitivity:** The sensitivity of this method is 0.140-0.180 absorbance units per g/dL.

**Linear Range:** In this method there is no significant nonlinearity over the range of 0-6 g/dL.

**Precision:** Within-run and day-to-day precision is summarized below.

Albumin |
Within-Run |
Total |

Mean |
SD |
CV |
SD |
CV |

g/dL |
g/dL |
% |
g/dL |
% |

1.2 |
0.04 |
3.50 |
0.05 |
4.30 |

3.4 |
0.05 |
1.40 |
0.07 |
2.00 |

5.6 |
0.06 |
1.10 |
0.06 |
1.00 |

##
Correlation

A comparison of this method using an automated analyzer and a reference method based upon the BCG reaction resulted in the following regression statistics.

Correlation |
Data |

Parameter |
Data Observed |

N |
197 |

Range |
1.6-5.2 g/dL |

Regression |
Y = 1.039x - 0.12 |

Correlation |
r = 0.999 |

S_{y,x} |
0.09 |