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DISCRETPAK™REAGENTS - Albumin

Intended Use

For IN VITRO quantitative determination of Albumin in serum or plasma using manual or automated applications.

Method Principle

BCG dye at a pH of 4.2 and in the presence of a non-ionic surfactant Brij-35 specifically binds albumin to form a colored BCG-Albumin complex with a maximum absorbance at 630nm. The increase in absorbance is directly proportional to the Albumin concentration present in the sample.

pH 4.2
                 Albumin + BCG  ──────> BCG-Alubmin Complex
Brij-35

Method Performance Characteristics

Sensitivity: The sensitivity of this method is 0.140-0.180 absorbance units per g/dL.
Linear Range: In this method there is no significant nonlinearity over the range of 0-6 g/dL.
Precision: Within-run and day-to-day precision is summarized below.

Albumin Within-Run Total
Mean SD CV SD CV
g/dL g/dL % g/dL %
1.2 0.04 3.50 0.05 4.30
3.4 0.05 1.40 0.07 2.00
5.6 0.06 1.10 0.06 1.00

Correlation

A comparison of this method using an automated analyzer and a reference method based upon the BCG reaction resulted in the following regression statistics.

Correlation Data
Parameter Data Observed
N 197
Range 1.6-5.2 g/dL
Regression Y = 1.039x - 0.12
Correlation r = 0.999
Sy,x 0.09