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VETSPEC™REAGENTS - Total Protein

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Total Protein in serum using manual or automated applications.

Method Principle

A serum sample is mixed with the biuret reagent. The carboxyl (-C=0) and imine (=N-N) groups of the peptide bond present in the protein molecular structure react with the Copper ions under alkaline conditions  to form a colored Chelate complex. The intensity of the color produced is read at 550 nm and it is directly proportional to the Total Protein concentration in the serum sample. The reaction scheme below illustrates the reactions that occur in this method:

Protein (-C=0; =N-N) + Biuret (Cu2+) ────> Chelate Complex


Preparation Of Working Reagents

Catachem Total Protein reagents are packaged ready to use. No preparation is required.

Reagent Storage And Stability

Store Catachem Total Protein reagents at room temperature (18-26°C). When stored as directed, the reagents are stable until the expiration date stated on the label.

Method Performance Characteristics

Sensitivity: 0.0336 - 0.0410 absorbance units per g/dL.
Linear Range: 0-10g/dL.
Precision: Within-run and day-to-day precision is summarized below.

Total Protein

Within-Run Precision

Total Precision

MEAN

SD

CV

SD

CV

g/dL

g/dL

%

g/dL

%

2.80

0.10

3.40

0.10

3.50

5.90

0.11

1.80

0.12

2.10

8.70

0.11

1.20

0.17

3.00


Correlation

A comparison of this method using an automated clinical instrument was carried out against a reference biuret method resulted in the following regression statistics:

Correlation Data

Parameter

Data Observed

N

198

Range (g/dL)

3.9-10.0

Regression

Y = 0.92x + 0.48

Correlation

r = 0.982

Sy,x

0.19