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VETSPEC™REAGENTS - Uric Acid

Intended Use

For IN VITRO diagnostic use in the quantitative determination of Uric Acid in serum using manual or automated applications. p>

Method Principle

The Uricase enzyme catalyzes the oxidation of Uric Acid to produce Allantoin and Hydrogen Peroxide. The Hydrogen Peroxide formed is quantitated by oxidative coupling of 4-aminoantipyrine with 3-5 dichloro-2-hydroxybenzene sulfonic acid (DHBS) in the presence of Peroxidase. The intensity of the color produced is directly proportional to the concentration of the Uric Acid in the sample. The color complex formed is read at 500 nm. The reaction scheme below illustrates the reactions that occur in this method.

Uricase
             UA + O2 + 2H2O ────────> Allantoin + CO2 + H2O2

   Peroxidase
 H2O2 + DHBS + 4AA ────────> Quinonemine Dye


Preparation Of Working Reagent

Catachem Uric Acid reagent is packaged ready for use. No preparation is required.

Reagent Storage And Stability

Store the Catachem UA Reagent at 2-8°C. When stored as directed, this reagent is stable until the expiration date stated on the label. Once opened, keep reagent at 2-8°C and capped while not in use.

Method Performance Characteristics

Sensitivity: 0.014 - 0.018 absorbance units per mg/dL.
Linear Range: 0-20 mg/dL.
Precision: Within-run and day-to-day precision is summarized below.

Uric Acid

Within-Run Precision

Total Precision

MEAN

SD

CV

SD

CV

mg/dL

mg/dL

%

mg/dL

%

2.10

0.00

0.00

0.05

2.20

11.60

0.08

0.65

0.08

9.68

21.50

0.09

0.42

0.12

0.54


Correlation

A comparison of Catachem Uric Acid method using an automated analyzer and a reference Uric Acid method based upon the Uricase-Peroxidase reaction resulted in the following regression statistics:

Correlation Data

Parameter

Data Observed

N

124

Range (mg/dL)

2.5-15.1

Regression

Y = 0.991x + 0.198

Correlation

r = 0.997

Sy,x

0.23