For IN VITRO quantitative determination of Albumin in serum or plasma using manual or automated applications.
BCG dye at a pH of 4.2 and in the presence of a non-ionic surfactant Brij-35 specifically binds albumin to form a colored BCG-Albumin complex with a maximum absorbance at 630nm. The increase in absorbance is directly proportional to the Albumin concentration present in the sample.
Sensitivity: The sensitivity of this method is 0.140-0.180 absorbance units per g/dL.
Linear Range: In this method there is no significant nonlinearity over the range of 0-6 g/dL.
Precision: Within-run and day-to-day precision is summarized below.
A comparison of this method using an automated analyzer and a reference method based upon the BCG reaction resulted in the following regression statistics.
|Regression||Y = 1.039x - 0.12|
|Correlation||r = 0.999|